Pyrimidine Dimer Excision Repair of DNA in Bacteroides fragilis Wild-type and Mitomycin C-sensitive/UV-sensitive Mutants
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چکیده
منابع مشابه
Isolation of Chinese hamster ovary cell mutants deficient in excision repair and mitomycin C bioactivation.
Mitomycin C (MMC), a bifunctional alkylating agent, requires metabolic reduction to become biologically active. We have identified a series of genetically related Chinese hamster ovary cell lines which span approximately three orders of magnitude in the concentration of MMC required for cell killing. Many mechanisms, including drug transport, drug activation, drug detoxification, and the elimin...
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A putative transcriptional regulator of the AraC/XylS family was identified in a genomic genebank of Bacteroides fragilis Bf-1, which partially relieved the sensitivity of Escherichia coli DNA repair mutants to the DNA-damaging agents, metronidazole and mitomycin C. A homologue of this gene with the same phenotype was identified as BF638R3281 in B. fragilis 638R. Transcription of BF638R3281 was...
متن کاملAtomic model of a pyrimidine dimer excision repair enzyme complexed with a dna substrate: Structural basis for damaged DNA recognition
T4 endonuclease V is a DNA repair enzyme from bacteriophage T4 that catalyzes the first reaction step of the pyrimidine dimer-specific base excision repair pathway. The crystal structure of this enzyme complexed with a duplex DNA substrate, containing a thymine dimer, has been determined at 2.75 A resolution. The atomic structure of the complex reveals the unique conformation of the DNA duplex,...
متن کاملCharacteristics of excision repair of pyrimidine dimers in eukaryotic cells as assayed with anti-dimer sera.
An antiserum that recognizes the three types of pyrimidine dimers induced by UV-light in DNA was used to monitor their removal from eukaryotic cell nuclei. Cells able to excise dimers display a similar removal pattern: a fast process completed within a few hours leaving many dimers in the DNA.
متن کاملRapid and sensitive assay for detection of enterotoxigenic Bacteroides fragilis.
Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and cytotoxic response in HT29/C1 cells. By using a pair of monoclonal antibodies ...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1986
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-132-9-2577